Most of the above described effects were also observed in plants that overexpressed glycolate dehydrogenase alone. Leaf fresh and dry weight measurements revealed that total plant productivity might be enhanced. PAPER Mitochondrial glycolate oxidation contributes to photorespiration in higher plants Markus Niessen, Krishnaveni Thiruveedhi, Ruben Rosenkranz. Moreover, leaves of transgenic plants expressing the glycolate pathway showed higher glucose and fructose, end products of photosynthesis. The CO2 assimilation rates in transgenic plants were enhanced under photorespiratory conditions. Additionally, the establishment of the bacterial glycolate pathway in plant chloroplasts results in a decrease of the CO2 compensation point (Gamma). A further reduction of the photorespiratory flow was observed when all the transgenes were expressed in one plant (GTA). Thupakula Krishnaveni Nemali Sudha 9490893783 1-461 new indhira nagar Sangitham Sugunamma 9000949856 1-463-B indira nagar VEERAMALLI KRISHNAIAH 8977511858 461-46 INDIRA NAGAR Tupili Chengamma 1/461-223-B indira nagar Shaik Basha 9346794678 1-2-134 indira nagar Shaik Jilanibasha 8985832024 V PADMA 1-461-384-A INDIRA NAGAR KOMMI RATHNAMMA. By measuring the postillumination burst (PIB), a clear reduction in photorespiration was determined in plants transgenic for AtGDH. Various physiological, biochemical and photosynthetic measurements were performed under ambient and enhanced photorespiratory conditions to evaluate the impact of the established pathway in planta. The expression level of the transgenes was analyzed by RT-PCR and the respective enzymatic activity assays showed that the proteins are active in planta. tabacum plants containing the necessary genes were generated. coli GDH, the glycolate dehydrogenase from Arabidopsis thaliana (AtGDH) was used. Three enzymatic activities are required: glycolate dehydrogenase (GDH), glyoxylate carboligase (GCL), and tartronic semialdehyde reductase (TSR). coli and converts the glycolate formed during photorespiration into glycerate. Markus Niessen Krishnaveni Thiruveedhi Ruben Rosenkranz Rashad Kebeish Heinz-Josef Hirsch Fritz Kreuzaler Christoph Peterhänsel J Exp Bot 2007 26 58(10):2709-15. The new pathway aims at increasing the refixation of CO2 inside the chloroplasts and thereby at suppressing photorespiration in C3 plants. Kebeish, Markus Niessen, Krishnaveni Thiruveedhi, Rafijul Bari, Heinz-Josef Hirsch, Ruben Rosenkranz, Norma Stbler, Barbara Schnfeld, Fritz Kreuzaler and Christoph Peterhnsel. In the present study, an alternative biochemical pathway for the metabolism of glycolate was established in the chloroplasts of tobacco (Nicotiana tabacum) plants.
The photorespiratory pathway in C3 plants consumes not only ATP and reducing equivalents but also results in loss of ~ 25% carbon that has been fixed during the process of photosynthesis.
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